Figure 5.

CEES-induced activation of caspase-3 and inhibitory effects of thiol antioxidants in Jurkat cells. Cells were incubated first for 1 h with or without 5 mM NAC, 1 mM GSH-ethyl ester, or 5 mM 3-AB, and then for 12 h in the additional absence or presence of 600 μM CEES. Cell extracts were then prepared and assayed for caspase-3-like activity. Data are expressed in arbitrary units, and are from an experiment that was repeated a total of three times with similar results.