Table 2.
Effects of various treatments on the vasorelaxation responses to NADA in third-order branches (G3) of the rat superior mesenteric artery
| pEC50 | Rmax | n | |
|---|---|---|---|
| Control | 6.39±0.12 | 79.1±4.4 | 10 |
| KCl contracted vessels | 6.37±0.16 | 28.1±2.1 | 4 |
| SCH23390 (1 μM) | 6.34±0.22 | 65.8±6.9** | 7 |
| L-NAME (300 μM) | 6.45±0.14 | 85.3±5.4 | 6 |
| Endothelium denuded | 5.70±0.29** | 39.1±4.7** | 6 |
| ChTx (100 nM) & apamin (500 nM) with L-NAME (300 μM) & indomethacin (10 μM) | 5.73±0.29** | 40.0±7.0** | 5 |
| Capsazepine (10 μM) | 5.37±0.11** | 78.7±6.3 | 5 |
| Capsaicin pretreatment (10 μM) | 6.05±0.17** | 60.3±6.4** | 10 |
| Capsaicin pretreatment (10 μM) & L-NAME (300 μM) | 5.51±0.16** | 62.8±5.5** | 6 |
| SR141716A (100 nM) | 6.73±0.21 | 75.3±6.8 | 5 |
| SR141716A (1 μM) | 5.56±0.17** | 63.8±7.0** | 6 |
| Endothelium denuded & SR141716A (1 μM) | 5.78 ± 0.28** | 47.1±7.8** | 6 |
| Endothelium denuded & capsaicin pretreatment (10 μM) | 5.17±0.14** | 90.0±10.4 | 6 |
| Endothelium denuded & capsaicin pretreatment (10 μM) & SR141716A | 4.91±0.14** | 87.9±11.5 | 6 |
| Capsaicin pretreatment (10 μM) & SR141716A (1 μM) | 5.02±0.34** | 54.1±16.2** | 6 |
| AM251 (100 nM) | 6.86±0.30 | 65.8±8.3** | 6 |
| AM251 (1 μM) | 6.73±0.21 | 69.8±6.4 | 6 |
| O-1918 (1 μM) | 5.26±0.20** | 69.8±10.8 | 6 |
Data are given as means±s.e.m. Significant inhibitory effects compared with control vasorelaxant responses are indicated by *. * P<0.05.
**
P<0.05, as determined by ANOVA.