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. 2006 Sep 2;3:22. doi: 10.1186/1742-2094-3-22

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Copyright © 2006 Canova et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Immunofluorescence analyses of the human tumour-derived ependymoma primary cultures (clone 9945) stained for complement regulatory proteins and cell markers. Cells on coverslips were fixed with acetone and stained with antibodies against ependymal cells specific markers (GFAP, ZO-1 and S100, not shown) and complement regulators proteins (CD55, CD59, CD46 and CD35). Original magnification ×400. Background staining was observed using irrelevant antibodies (inset). Nuclei were counterstained with DAPI (blue).