Table 5.
Comparison of amino acid usage between two clusters of probable membrane associated proteins and between potential highly and lowly expressed genes
| N. equitans | ||||||||
| Amino acids | Upper cluster genes | Lower cluster genes | Highly expressed genes | Lowly expressed genes | ||||
| Number | (%) | Number | (%) | Number | (%) | Number | (%) | |
| Phe | 771 | 7.88* | 649 | 4.20 | 367 | 3.55 | 402 | 5.20 |
| Leu | 1708 | 17.46* | 1394 | 9.01 | 954 | 9.24 | 912 | 11.81 |
| Ser | 626 | 6.40 | 1060 | 6.85 | 383 | 3.71 | 409 | 5.30 |
| Tyr | 585 | 5.98 | 1117 | 7.22+ | 429 | 4.15 | 494 | 6.40 |
| Cys | 33 | 0.34 | 424 | 2.74+ | 46 | 0.45 | 85 | 1.10 |
| Trp | 102 | 1.04 | 137 | 0.89 | 95 | 0.92 | 77 | 1.00 |
| Pro | 329 | 3.36 | 597 | 3.86 | 488 | 4.72 | 314 | 4.07 |
| His | 96 | 0.98 | 118 | 0.76 | 155 | 1.50 | 61 | 0.79 |
| Gln | 155 | 1.58 | 347 | 2.24+ | 243 | 2.35 | 137 | 1.77 |
| Arg | 178 | 1.82 | 345 | 2.23 | 444 | 4.30 | 204 | 2.64 |
| Ile | 1373 | 14.04* | 1604 | 10.37 | 866 | 8.38 | 940 | 12.17 |
| Met | 190 | 1.94* | 168 | 1.09 | 226 | 2.19 | 128 | 1.66 |
| Thr | 426 | 4.35 | 879 | 5.68+ | 484 | 4.69 | 311 | 4.03 |
| Asn | 405 | 4.14 | 1384 | 8.95+ | 446 | 4.32 | 459 | 5.94 |
| Lys | 627 | 6.41 | 1504 | 9.72+ | 1099 | 10.64 | 840 | 10.88 |
| Val | 571 | 5.84 | 864 | 5.59 | 836 | 8.09 | 389 | 5.04 |
| Ala | 558 | 5.70* | 600 | 3.88 | 703 | 6.81 | 344 | 4.45 |
| Asp | 267 | 2.73 | 724 | 4.68+ | 516 | 5.00 | 327 | 4.23 |
| Glu | 341 | 3.49 | 816 | 5.28+ | 895 | 8.66 | 508 | 6.58 |
| Gly | 441 | 4.51 | 737 | 4.76 | 655 | 6.34 | 383 | 4.96 |
Values marked with *or + are significantly (p < 0.001) more frequent in upper or lower cluster gene products respectively and the values in bold faces indicate significant (p < 0.001) overrepresentation of corresponding amino acids in potential highly expressed genes.