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. 2006 Aug 31;7:223. doi: 10.1186/1471-2164-7-223

Table 1.

Sequences of PtMPKs 3'RACE and RTqPCR primers used for 3'UTR MAPK gene isolation and expression profiling.

Group Gene name 3' RACE Primers (5' to 3') Forward RTqPCR Primers (5' to 3') Reverse RTqPCR Primers (5' to 3')
A PtMPK3-1 CCCACTTGTTCACCCTCTGGCCATTG CCATAAGCACTACCAGTCC TCA GATACAGAGGAGTGC
PtMPK3-2 AGCATTGGCTCATCCATACCTTGCAAGG CTTTAGCACTACCAATCCCG TGAACAATTAGCAAGTCCAGG
PtMPK6-1 TCCCAACTGTCCACCCGGCAGCTATTG CATTGTGACTGGTCAGCTTGA GTTCCCCTGAATTCTGTCC
PtMPK6-2 GATCCCAGACAGAGGATTACTGTTGAGG ATGAGTCGACCAGCGTGGC CACTACATTGACGCCGATAC
B PtMPK4 AGTCGATGAGGCACTGTGCCATCCATAC TGGTGTGCAGTTGGGAAGG GAACCTTCATAGAGATTCTGC
PtMPK5-1 TCGCATCACTGTTGATGAGGCCCTTTGT CGTCATTTCCTGGAGAATATG ACAATGCAATTCACTTCAGGC
PtMPK5-2 CCCAATAACCGCATCACTGTCGATGAGG CATAACTTCTGACACATTTGGG CATACAACACATGATCCTTGC
PtMPK11 GAGGCATTGTGCCATCCATACTTGGCAC GTTTGCTGAGCAGAATCTCC GCGTCCTCAAAACCATGAAG
C PtMPK1 CCGGAGCACTGGAACACCCTTACATGTC AAGAAGCACGCACAAGCCAG TACAAGCTGAAAGGGCCACC
PtMPK2 GCTGCAACCCTCCAGCTCAGGTCCC GTGTGTTTTTGTACGTGTCTG AGAACTAGAAGCTCACTGCC
PtMPK7 CAGGGCTGTACGATCCAAGACGCGACCC CTAGTGTGTCTGTGAGATCAG GGATGCCATAGTCCGGCTC
PtMPK14 ACCCTTACATGTCAGGGCTGCACGATCC TGCACGATCCAAGACACGAC TGCGAAAACAACTTCTGGGTG
D PtMPK9-1 CCAGGGTCACAGCAACCAGATGGTTCAG GATTCAGATGGCTGCCTTTG ACAAGCTACTGCCTGATCTTC
PtMPK9-2 GCAGCTCAAAGCACTGATATTGAGAGGC CCAGCGTAGTGTGGATAGTG TCACATCTCCTCTCTGCAATC
PtMPK16-1 CGGTGCTGCGTTACAACAATTGTGGAGC AATCTGTGCCATTTTGTCTGTG TGGCCTTATGAATACAGCCAC
PtMPK16-2 GTGGAGCAGCAGCAGCAGAGAATCTTGA CAGCAGCAGAGAATCTTGAC CCATTGGAACCTTCAACCAC
PtMPK17 CTCTTTCTCGTGCAGCTAAGCAGAGCCC CCTATTTGCACATCTTGGAGG GCGGTAGTACATTTAGTGAGG
PtMPK18 CCTCCCCAAACCAGCTCTCCACACCACTGCT GATAAATCCTTTCCACCCTCG GTTGACTACACCCTTGAAACC
PtMPK19 CTGGGATGGCCATGGATGTGAACGCCTA GTTGGTATCACGATCAATTATG TGACTAGATACAGGACGTGG
PtMPK20-1 GGGAGGGAGCAAGGAAGAACATGTGGAT GGACTGAGGAGATCCACTTG CCCCCTCTTTTTACACAATCC
PtMPK20-2 GTGGCATGGCAGCCAAATATGCACCAGA GGTTGGAGCTGTTCAATATGG GACACGTTGATCTTCTGAGTC