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. 2006 Aug 31;7:223. doi: 10.1186/1471-2164-7-223

Table 2.

Sequences of PtMKKs " 3'RACE and RTqPCR primers used for 3'UTR MAPKK gene isolation and expression profiling.

Group Gene name 3' RACE Primers (5' to 3') Forward RTqPCR Primers (5' to 3') Reverse RTqPCR Primers (5' to 3')
A PtMKK2-1 TGGTATTGCTGGAGTGTGCAACAGGCCA TGCAAATGCATCAAACTCCTG CCACCCAAAAGAGACATCAG
PtMKK2-2 CTCCACCTTCTGCACCACCAGACCAATT AGCTTGCAAACTATGAGGAAC CGATTCTGTCAGGGAAAACC
PtMKK6 GCTGCAACCCTCCAGCTCAGGTCCC GGGAAGGTTGTCATCTTTGG TGGGTAATTCACAGGAGGTTC
B PtMKK3 GCAGTTGCAATCCGCGTTTCAGGATCC GCTCTATGTTCCTCCAGTTAC GAAATGGTTTCATATCTGTCACG
C PtMKK4 ATTGCTTGTTGTTTGCAGAGGGAGCCGG GAATTGTTGTGGGTTATTGTTG GGTCAGAACTAAAGGTGCTGTG
PtMKK5 CCCAATAACCGCATCACTGTCGATGAGG AGCATCCGTTTATTGTGAGAAG AACTACCTACTAAGCAATTGGC
D PtMKK7 CGAGCTTGCCGGAGGGAGCATCTGAGGA TGACCCGAATGCCCAGTTAA TTCCTCTCATTTCAAGAGCAG
PtMKK9 GCGTCGGAGGAGTTTCGGGACTTTATTC ACTTTATTCAGTGTTGCCTGC GGGTCAAATCACAAGTCCAC
PtMKK10 TGTGGAGAGAAACCAGACTGGGCAGCAT TTTGGTCTGGATATAAGCTGTG TGCAAAACCAGCATCATCTTC
PtMKK11-1 GGGAGTGCTCTGCAGTTGTTGCAACACC TGCAACACCCTTTTATACTGC TGATGGAAGTGACAGGATGG
PtMKK11-2 GGAGAATCCCCTAGCTTCCCAAAGGAAG CCTATGTTTGCTTAGGGGAG TTGGTCCAAGAGCCATTTCG