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. 2006 Sep 22;2(9):e97. doi: 10.1371/journal.ppat.0020097

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© 2006 Chaput et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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PG from parental strain 26695 (A) and its amiA isogenic mutant (B) were purified and digested with the muramidase M1 (mutanolysin). The generated muropeptides were separated by HPLC. The HPLC profiles show muropeptide composition after 8 h, 24 h, and 48 h of bacterial growth. Each peak structure was assigned by MALDI-TOF mass spectrometry and corresponds to a different muropeptide: (1) GM-tripeptide, (2) GM-tetrapeptide, (3) GM-tetrapeptide-glycine, (4) GM-dipeptide, and (5) GM-pentapeptide. Dimers were then eluted: (6) GM-tetrapeptide-tripeptide-MG, (7) GM-tetrapeptide-tetrapeptide-glycine-MG, (8) GM-tetrapeptide-tetrapeptide-MG, and (9) GM-tetrapeptide-pentapeptide-MG. Finally, anhydromuropeptides were eluted: (10) G(anh)M-pentapeptide, (11) and (12) G(anh)M-tetrapeptide-tripeptide-MG, (13) and (14) G(anh)M-tetrapeptide-tetrapeptide-MG, and (15) G(anh)M-tetrapeptide-pentapeptide-MG.