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. Author manuscript; available in PMC: 2006 Sep 25.
Published in final edited form as: FASEB J. 2006 Jul;20(9):1336–1344. doi: 10.1096/fj.06-5734com

Figure 2.

Figure 2

Study design. Healthy men and women were fed a baseline diet containing the choline Adequate Intake concentration for 10 days. They were then switched to a low choline diet (<50 mg choline) until they developed signs of organ dysfunction associated with choline deficiency or for up to 42 days. Subjects who developed signs of organ dysfunction were repleted with graded amounts of choline at 10 day intervals until their symptoms disappeared; those without signs of organ dysfunction were fed the 100% choline diet for at least 3 days before being discharged from the study. Some subjects were given a folic acid supplement (400 μg per day) during the depletion and repletion phases, but this did not affect their susceptibility to choline deficiency (from da Costa et al., Am J. Clinc. Nutr., in press).