TABLE 2.
Primers for sequencing the PEMT promoter regiona
| Position of primer (bp) | Primer name | Sequence of primer 5′→3′ |
|---|---|---|
| 7728 - 7757 | PEMT PRO - F3 | GGAGTTATGGATCTAGGGAACTGGAGCAGC |
| 7711 - 7730 | PEMT PROseq - F1 | ATTTCACCCTCCTGAAAGGA |
| 8102 - 8082 | PEMT PROseq - R1 | TGACCAATCTAAGCCCAGGTT |
| 8092 - 8112 | PEMT PROseq - F2 | TAGATTGGTCATGGGAGGCTT |
| 8493 - 8474 | PEMT PROseq - R2 | ACAACATGGTGACACTCCGT |
| 8503 - 8522 | PEMT PROseq - F3 | TCTCGAACTCCTGACCATCA |
| 8878 - 8860 | PEMT PROseq - R3 | CCCGTAATCCCAGCACTTT |
| 8915 - 8935 | PEMT PROseq - F4 | GAGGAAAAAGACTCTGGCACA |
| 9300 - 9280 | PEMT PROseq - R4 | TTTACTCCATTGAGGGGTGCA |
| 9084 - 9101 | PEMT PROseq - F5 | TGATGGATCCCAGGAGGA |
| 9510 - 9490 | PEMT PROseq - R5 | GGCTTTCTGCTACCCAGTAAT |
| 9525 - 9498 | PEMT PRO - R1 | ACAACAGAGGTCCCGGCTTTCTGCTAC |
| 8438 - 8458 | PEMT PROseqMid - F3 | ACAACAGAGGTCCCGGCTTTCTGCTAC |
| 8824 - 8802 | PEMT PROseqMid - R3 | TCAGAGATCAGCCTGGCCAATAT |
| 8461 - 8480 | PEMT PROseqMid - F4 | ATTTTTAGTAGAGACGGAGT |
| 8840 - 8821 | PEMT PROseqMid - R4 | ATCACAAGGCCAGGAGTCAG |
| 8374 - 8394 | PEMT PRO SNP1- F | ACTTCCTGGGTTGAAGCGATT |
| 8597 - 8579 | PEMT PRO SNP1- R | TTTATTCTCTGGCCGTGCC |
a
Primers were used to sequence the PEMT gene as described in Materials and Methods.