Figure 2.
Inhibition of specific [3H]-CP55.940 binding (20 pM) by CP55.940, AM251, Δ9-THC, WIN55212-2 (a, c) and anandamide±PMSF (50 μM; b, d) in human neocortical (upper panel) and amygdala (lower panel) synaptosomes. Synaptosomes (∼100 μg protein) were incubated for 60 min at 30°C with radiolabelled CP55.940 and various concentrations of drugs under study. Nonspecific binding was determined by using 10 μM CP55.940. The data points of each curve are means±s.e.m. of four experiments (i.e. tissue of 4–7 different patients), each performed in quadruplicate.