Figure 3.

Effect of AH 6809 on the inhibition of IL-1β-induced GM-CSF evoked by PGE₂, 16,16-dimethyl PGE₂ and ONO-AE1-259. Adherent HASM cells were pretreated (30 min) concurrently with indomethacin and AH 6809 (both 10 μM) and then for a further 5 min with PGE₂ (a), 16,16-dimethyl (b) or ONO-AE1-259 (c). IL-1β (100 pg ml⁻¹) was then added to the cells and the GM-CSF released into the culture medium was quantified at 24 h by a sandwich ELISA. Each data point represents the mean±s.e.m. of three to five determinations using tissue from different donors.