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In vitro phosphorylation of purified IR in the presence or absence of peptide (LPFFD, KIQMK, DIYET, KIFMK, or KIYEK). Purified IR was incubated in buffer with or without 100 nM insulin, and with or without peptide for 10 min at 37°C. Four results of each peptide displayed on the top panel represent typical immunoblots. *P<0.05, **P<0.01 vs insulin-stimulated tyrosine phosphorylation without peptide, n=4 for each lane.
