Figure 5.

Effect of lignocaine on in vitro insulin-stimulated tyrosine phosphorylation of IR at different time points. Purified IRs were incubated in buffer containing 100 nM insulin for 0, 10, 20, or 30 min at 37°C, respectively. One point was made for each time, resulting in different incubations with or without lignocaine. Lignocaine was added at 11 min after insulin stimulation in the samples of 20 or 30 min incubation. Results displayed on the top panel represent typical immunoblots. ^**P<0.01 vs insulin-stimulated tyrosine phosphorylation at 10 min incubation, n=4 for each lane.