Figure 4.

Effects of 1 μM raloxifene on Ca²⁺ transient kinetics. (a) Bar graphs showing the effects of raloxifene (1 Ral) on the time-to-peak indo-1 ratio (TTP) and time-to-50% Ca²⁺ transient decay (R50) in freshly isolated myocytes (left) and in myocytes that have been preincubated with 10 μM ICI 182,780 for 1 h (right). (n=14, ^***P<0.001). (b) Representative traces of Ca²⁺ transients in normal tyrode (NT, grey traces) and in the presence of raloxifene (black traces). Traces have been normalised to the same amplitude to facilitate comparison. Raloxifene can be seen to prolong TTP in the myocyte on the left, but not in the myocyte preincubated with ICI 182,780 on the right.