Figure 6.

Effects of 18 h culture with nateglinide on nateglinide-, tolbutamide-, glibenclamide- or efaroxan-stimulated insulin secretion. BRIN-BD11 cells were cultured for 18 h in either standard tissue culture medium, or in tissue culture medium supplemented with 100 μM nateglinide. Following 40 min preincubation with a buffer containing 1.1 mM glucose, effects of 200 μM nateglinide, tolbutamide, glibenclamide or efaroxan were tested during a 20 min incubation period. Values are mean±s.e.m. (n=6). ^***P<0.001 compared with the respective effect in the absence of drug. ΔΔΔP<0.001 compared with the respective effect in the absence of nateglinide during 18 h culture.