Table 5.
Sensitivity of various probes to different reactive species
| Reactive species | HPF | APF | DCFH |
|---|---|---|---|
| OHa | 730 | 1200 | 7400 |
| ONOO−b | 120 | 560 | 6600 |
| Hypochloritec | 6 | 3600 | 86 |
| Singlet O2d | 5 | 9 | 26 |
| Superoxidee | 8 | 6 | 67 |
| H2O2f | 3 | <1 | 190 |
| NO•g | 6 | <1 | 150 |
| ROO•h | 17 | 2 | 710 |
| Autoxidationi of the probe | <1 | <1 | 2000 |
Fluorescence probe reagents were added to sodium phosphate buffer (0.1 M, pH 7.4) (final 10 μM; 0.1% DMF as a cosolvent). The fluorescence intensities of HPF, APF and DCFH were measured at excitation wavelength of 490, 490 and 500 nm and fluorescence emission wavelength of 515, 515 and 520 nm, respectively.
100 μM of ferrous perchlorate (ll) and 1 mM of H2O2 were added.
3 μM (final) of ONOO− was added.
3 μM (final) of NaOCI was added.
100 μM of 3-(1,4-dihydro-1,4-epidioxy-1-naphthyl)propionic acid was added.
100 μM of KO2 was added.
100 μM of H2O2 was added.
100 μM of 1-hydroxy-2-oxo-3-(3-aminopropyl)-3-methyl-1-triazene was added.
100 μM of 2,2′-azobis(2-amidinopropane)dihydrochloride was added.
Fluorescence probe reagents solutions were placed under a fluorescent lamp for 2.5 h.
Adapted from Setsukinai et al. (2003).
We thank Professor Tetsuo Nagano and the American Society of Biochemistry and Molecular Biology for granting permission to reproduce this table.