Figure 6.

G-protein-coupled receptor dependence of WIN-2 inhibition of 50 mM K⁺-evoked CGRP release: 10 μM WIN-2 inhibition of 50 mM K⁺-evoked CGRP release was assessed in the presence of the CB1 antagonists, SR141716A (a) and AM251 (b), as well as the CB2 antagonist, AM630 (c), n=6, all experiments, ^***P<0.001 vs K⁺ alone). (d) The effect of G_i/o G protein uncoupling was tested by 18 h pretreatment with PTX (n=6, ^*P<0.05 vs K⁺ alone). All data are presented as the sum of the agonist±antagonist pretreatment+the K⁺-evoked CGRP release treatment to account for the ability of WIN-2 to evoke CGRP release on its own.