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Influence of complete replacement of sodium (triangles) by choline (squares) on the inhibition of [3H]GR65630 binding to intact N1E-115 cells by 5-HT. The 5-HT effects are expressed as percentages of [3H]GR65630 binding (=control) after 60 min exposure to 1 nM of the radioligand in the absence of 5-HT. Under both buffer conditions, two components with two different IC50 values could be calculated. Shown are means±s.e.m. of n=4 experiments.
