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Noladin ether is a weak agonist at hTRPV1. Intracellular Ca2+ concentration was monitored using Fluo-3 in human vanilloid TRPV1 receptor-expressing HEK293 cells before and after the addition of capsaicin (100 pM–30 μM), anandamide (100 pM–10 μM) or noladin ether (100 pM–10 μM). Responses were measured as peak increase in fluorescence minus basal and are given as mean±s.e.m. n=3.
