Figure 6.

Agarose-gel electrophoresis showing PCR products obtained when using specific primers for selected α₁- and β-subunits of voltage-dependent Ca²⁺ channels and cDNA from mesenteric arterioles. The Ca_V2.1-P and Ca_V2.1-Q are alternative splice variants of the same gene and corresponds to the P-type and Q-type α₁-subunits, respectively. Ca_V3.1-T corresponds to the α_1G-subunit of T-type channels. β-2 (cardiac), β-3 (smooth muscle), and β-B (β_1b, neuronal) are known β-subunits of high-voltage-activated Ca²⁺ channels. Specific primer for β-actin was also included in the reactions. Pooled cDNA from mesenteric arterioles in each of two rats were used (Prep 1, Prep 2). Appropriate controls are shown in which cDNA was omitted (neg. control) or 50 ng template cDNA isolated from various rat organs (cerebral cortex, left cardiac ventricle, or renal inner medulla) was included in the PCR (pos. control).