Table 3.
The fold change in kcat and KM for RF1 at codons differing from the RF1 stop codons (UAG and UAA) by 1 nt: The contribution to accuracy from kcat and KM
| Codon | Fold change in the kcat and KM parameters
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| −RF3
|
+RF3
|
|||||||||||
| kcat | (σ) | KM | (σ) | kcat | (σ) | KM | (σ) | |||||
| AAA | * | * | * | * | ||||||||
| AAG | * | * | * | * | ||||||||
| GAA | * | * | 790 | 40 | 500 | 200 | ||||||
| GAG | * | * | 700 | 300 | 900 | 500 | ||||||
| CAA | 180 | 10 | 2,000 | 400 | 53 | 1 | 1,600 | 300 | ||||
| CAG | 160 | 10 | 3,000 | 1,000 | 52 | 2 | 1,700 | 200 | ||||
| UGA | 57 | 5 | 2,400 | 300 | 20 | 1 | 1,900 | 100 | ||||
| UGG | 38 | 2 | 1,250 | 70 | 15.2 | 0.8 | 760 | 30 | ||||
| UCA | 14.7 | 0.2 | 1,000 | 100 | 7.0 | 0.2 | 600 | 30 | ||||
| UCG | 19.3 | 0.8 | 990 | 60 | 8.6 | 0.3 | 600 | 50 | ||||
| UUA | 23 | 1 | 1,200 | 200 | 9.67 | 0.06 | 780 | 40 | ||||
| UUG | 12 | 1 | 1,060 | 50 | 5.3 | 0.7 | 690 | 10 | ||||
| UAC | 17.2 | 0.3 | 1,180 | 60 | 8.2 | 0.6 | 700 | 100 | ||||
| UAU | 2.5 | 0.1 | 400 | 100 | 2.1 | 0.2 | 170 | 70 | ||||
The results are the mean of three experiments with SD of the mean (σ). Unresolvable values are represented by *; see legend to Table 1. Bases in bold are those that differ from the sequence of a normal stop codon.