Table 4.
The fold change in kcat and KM for RF2 at codons differing from the RF2 stop codons (UAG and UAA) by 1 nt: The contribution to accuracy from kcat and KM
| Codon | Fold change in the kcat and KM parameters
|
|||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| −RF3
|
+RF3
|
|||||||||||
| kcat | (σ) | KM | (σ) | kcat | (σ) | KM | (σ) | |||||
| AAA | * | * | * | * | ||||||||
| AGA | 1,300 | 100 | 290 | 20 | 480 | 30 | 370 | 30 | ||||
| GAA | 3,200 | 500 | 260 | 70 | 2,000 | 300 | 200 | 30 | ||||
| GGA | 540 | 20 | 290 | 30 | 300 | 20 | 280 | 20 | ||||
| CAA | 90 | 10 | 440 | 40 | 50 | 10 | 350 | 90 | ||||
| CGA | 61 | 5 | 240 | 50 | 34 | 2 | 190 | 20 | ||||
| UUA | 200 | 100 | 120 | 40 | 140 | 50 | 130 | 50 | ||||
| UCA | 3.1 | 0.2 | 2,100 | 70 | 2.13 | 0.03 | 2,000 | 200 | ||||
| UAG | 7.2 | 0.7 | 250 | 30 | 4.4 | 0.2 | 240 | 20 | ||||
| UGG | 8 | 1 | 310 | 30 | 5.8 | 0.2 | 260 | 50 | ||||
| UAC | 30 | 10 | 500 | 100 | 33 | 5 | 350 | 40 | ||||
| UGC | 34 | 3 | 500 | 100 | 20 | 1 | 300 | 50 | ||||
| UAU | 80 | 10 | 500 | 100 | 46 | 9 | 400 | 90 | ||||
| UGU | 51 | 2 | 430 | 40 | 32 | 1 | 290 | 30 | ||||
The results are the mean of three experiments with SD of the mean (σ). Unresolvable values are represented by *; see legend to Table 1. Bases in bold are those that differ from the sequence of a normal stop codon.