Figure 6.

DNA binding activity of C/EBP in apicidin-, TSA- and n-butyrate-treated cells. (a) HL-60 clone 15 cells were incubated for the periods indicated in the presence of apicidin (100 nM), TSA (30 nM) or n-butyrate (500 μM) and the nuclear proteins were extracted. The amount of C/EBP in 5 μg of the nuclear proteins bound to the ³²P-labeled DNA probe containing the C/EBP binding site was detected by EMSA. (b) The nuclear extracts were preincubated with unlabeled-consensus probe, anti-C/EBPα (α), anti-C/EBPβ (β) or anti-C/EBPɛ (ɛ). The arrowheads indicate the complex of C/EBP with DNA (C/EBP-DNA), and C/EBP-DNA supershifted by the antibody (SS).