Figure 1.
Semiquantitative analysis of the expression of PPAR isoforms in rat islets and BRIN-BD11 cells. Panels a–c: RNA was extracted from rat islets (islet) BRIN-BD11 cells (Brin), rat liver and rat adipose tissue and amplified by semiquantitative RT–PCR (Taqman). Standard curves were constructed from liver (a, b) and adipose (c) and used to monitor the expression of PPAR isoforms (PPARα – panel a; PPARδ – panel b; PPARγ – panel c) in islet and BRIN-BD11 cells. The levels of expression of each PPAR isoform are expressed relative to that found in liver or adipose (defined as 1 in each case). Results are presented as relative expression±s.e.m. (n=3). Panel d: Proteins were extracted from adipose tissue (10 μg; lane 1), BRIN-BD11 cells (10 μg; lane 2), rat islets (5 μg; lanes 3–5), human islets (5 μg; lanes 6, 7) or HEK293 cells expressing PPARγ (5 μg; lane 8 – positive control) and separated by electrophoresis prior to transfer to PVDF membranes. The membranes were then probed with anti-PPARγ serum and immunoreactive bands were detected by chemiluminescence.