Figure 4.

Effect of denbufylline on cytokine generation from basophils. Cells were incubated for 15 min with denbufylline before challenge with anti-IgE (1 : 100,000 or 1 : 10,000) for 4 h for IL-4 generation (a) or 24 h for IL-13 generation (b). Values are expressed as the % inhibition of the control releases, which were for (a), 73±31 pg of IL-4 per 10⁶ basophils and for (b), 8±2 pg of IL-13 per 10⁶ basophils. Data for both IL-4 and IL-13 were generated from the same set of donors but on different days. Values are means±s.e.m., n=5. Asterisks denote; ^*P<0.05, ^**P<0.01.