Table 3.

Effects of Sp-8-CPT-cAMPS on cytokine generation and histamine release from basophils

	% inhibition
Stimulus	Histamine	IL-13	IL-4
Anti-IgE	64±5	80±7	81±7
IL-3	98±2	87±7	76±7

Cells were incubated for 30 min without or with Sp-8-CPT-cAMPS (1 mM) and then challenged with either anti-IgE (1 : 100,000) or IL-3 (100 ng ml⁻¹) for 24 h. After this time, supernatants were collected and assayed for histamine, IL-4 and IL-13 content. Values are expressed as % inhibition of the control releases, which were for anti-IgE, 20±3% histamine release, 34±7 pg IL-13 per 10⁶ basophils, 17±2 pg IL-4 per 10⁶ basophils and for IL-3, 15±4% histamine release, 856±307 pg IL-13 per 10⁶ basophils, 36±12 pg IL-4 per 10⁶ basophils. Sp-8-CPT-cAMPS inhibited the generation of all mediators, induced by both stimuli, to a statistically significant (P<0.05 at least) extent. Values are means±s.e.m., n=4–6.