Panel (a) Treatment with DETA-NO, 50 μM, prevents cleavage of procaspase-3 and -9 and PARP and attenuates cytochrome c translocation induced by APAP. Hepatocytes were incubated for 6 h as described in Methods. Lane 1: control cells. Lane 2: APAP, 6.6 mM, alone. Lane 3, APAP plus 50 μM DETA-NO. Each blot is representative of at least three other experiments Panel (b) DETA-NO protects from APAP-induced apoptosis. Data are mean±s.e. of six experiments. *P<0.05 versus control; **P<0.05 versus APAP alone.