Figure 5.

Effects of the treatment with Repertaxin or anti-CINC-1 on the increase in vascular permeability, recruitment of neutrophils and haemorrhage in the intestine and lung following severe ischaemia (120 min) and reperfusion (120 min) injury of the SMA. Changes in vascular permeability in the (a) intestine and (b) lungs were assessed by evaluating the extravasation of Evans blue dye. Neutrophil recruitment in the (c) intestine and (d) lungs was assessed by evaluating tissue levels of MPO. Haemorrhage was evaluating by haemoglobin content in the intestine (e). Repertaxin (30 mg kg⁻¹) was given i.v. 5 min prior to reperfusion, and the anti-CINC-1 antibody (aCINC-1) was given s.c. 60 min prior reperfusion. Control animals received saline (vehicle) or nonimune serum. Results are shown as μg Evans blue, as the number of neutrophils or μg haemoglobin per 100 mg of tissue and are the mean±s.e.m. of 5–6 animals in each group. ^*P<0.01 when compared to sham-operated animals; ^# P< 0.05 when compared to vehicle I/R animals.