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. 2004 Aug 2;143(1):143–151. doi: 10.1038/sj.bjp.0705897

Figure 1.

Figure 1

Albumin induces iNOS expression in RAW 267.4 cells. Nitrite production in RAW 267.4 cells after 24-h incubation with fraction-V type albumin (a), low-endotoxin albumin (b) and purified lipopolysaccharide (c) at given concentrations. n=5–8. Asterisks (a–c) indicate significant difference from the control without albumin or endotoxin incubation. (d) Western blots of iNOS expression after 24-h incubation with fraction-V type albumin and low-endotoxin albumin at given concentrations. (e) Human serum albumin (HAS, 10 mg ml−1) induces nitrite production in RAW 267.4 cells after 24-h incubation without inhibitors as well as in the presence of 1 μM cycloheximide (CH) or 100 μM NG-monomethyl-L-arginine (LN). n=3. (f) Nitrite production in freshly ex vivo prepared rat alveolar macrophages after 24-h incubation using media without albumin and inhibitors (ctrl), with media containing purified LPS (100 ng ml−1), low-endotoxin (LE) or fraction-V (FV) type of albumin (10 mg ml−1) and in the absence or presence of 1 μM cycloheximide or 100 μM NG-monomethyl-L-arginine. Asterisks (c, f) indicate significant difference from the level after stimulation with albumin or LPS in the absence of inhibitors.