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Effects of inhibition of different enzymatic phosphorylation pathways on albumin-induced NO production in RAW 267.4 cells. Cells were incubated with albumin (fraction-V type, 2 mg ml−1) for 24 h in the absence or presence of tyrphostin AG490 (AG), U0126 (U), SB203580 (SB) and pyrrolidine dithiocarbamate (PDTC), each at 30 μM. Asterisks indicate significant difference from the control (ctrl) value in the absence of inhibitors (P<0.05). n=4–6.
