Figure 3.
NO formation from PCA induced by repeated stimulation with 10 μM aspirin, 15 nM substance P, and 15 nM substance P after a single 15-min preincubation with 10 μM aspirin (n=4). After each stimulation, the aspirin- or substance P-containing incubation buffer was changed with test substance-free buffer. ***P<0.001 indicates a significant difference in NO response to aspirin compared to the initial aspirin-induced NO release (t=0). ++P<0.01, +++P<0.001 indicate significant differences in NO response to substance P after aspirin pretreatment compared to the respective value without aspirin pretreatment.