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. 2004 Aug 2;143(1):159–165. doi: 10.1038/sj.bjp.0705907

Figure 4.

Figure 4

(a) L-[3H]citrulline formation by EA.hy 926 cell homogenates in the L-arginine/L-citrulline conversion assay after 15 min incubation with 10, 100, 300, or 1000 μM aspirin and 100 μM aspirin concomitant with 1 mM L-NAME compared to the PBS control (n=4) (***P<0.001). (b) Incubation with 100 μM salicylic acid, or 100 μM of the acetylating compounds APHS or ASHA compared to 100 μM aspirin (n=4) (***P<0.001). (c) Incubation with 100 μM aspirin in normal calcium buffer and in nominally calcium-free buffer ([Ca2+]e=50 nM) compared to the respective PBS controls (n=4) (***P<0.001).