Figure 2.

Generation of O₂⁻/ONOO⁻ by donor drugs. (a) Representative EPR spectra showing the signal generated through oxidation of Tempone-H (1 mM) by O₂⁻/ONOO⁻ after incubation (30 min, 37°C) with SIN-1, GEA 3162 and DEA/NO (all 100 μM) in the presence or absence of SOD (500 U ml⁻¹). Control spectra of Iscove's medium and 1 mM Tempone-H have been subtracted from each trace. (b) Relative intensities (arbitrary units) for EPR signals generated over time in the presence of SIN-1, GEA-3162 or DEA/NO (100 μM) in the presence or absence of SOD (500 U ml⁻¹). Results are mean±s.e.m. (n=6).