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S1P1 receptor expression in all animal groups by immunofluorescence. Thin sections of mesentery (8–10 μm) were assessed by immunofluorescence for expression of von Willebrand factor (VWF), α actin, S1P1 receptor or a no primary antibody control. Tissues were obtained from young, intact aged. Ovx + Placebo aged or Ovx + Estrogen aged female rats.
