Table 1.
Maximal effects (Emax) of 5-HT for stimulation of cAMP accumulation, pEC50 values of 5-HT, 5-CT, 5-MeOT and 8-OH-DPAT and pA2 values of SB-269970 versus 5-CT in human glioblastoma cell lines
| Emax (5-HT) | pEC50 | pA2 | ||||
|---|---|---|---|---|---|---|
| Cell line | Fold basal | 5-HT | 5-CT | 5-MeOT | 8-OH-DPAT | SB-269970 |
| U-373 MG | 9.5±0.5 | 6.09±0.12 | 6.98±0.03 | 5.89±0.09 | <4 | 8.82±0.05 |
| DBTRG-05MG | 8.3±0.3 | 6.18±0.07 | 7.22±0.09 | 6.13±0.03 | <4 | 8.95±0.05 |
| T98G | 12.9±1.5 | 5.86±0.06 | 6.84±0.03 | 5.59±0.06 | <4 | 8.81±0.13 |
| H4 | 32.7±0.9 | 6.03±0.03 | 7.03±0.06 | 5.65±0.02 | <4 | 8.92±0.06 |
| U-138 MG | 7.6±1.5 | 5.69±0.04 | 6.58±0.08 | ND | ND | 9.05±0.07 |
| U-87 MG | 2.0±0.1 | 6.09±0.04 | 7.09±0.03 | ND | ND | 8.69±0.05 |
| CCF-STTG1 | 1.7±0.1 | 6.26±0.05 | 7.33±0.08 | ND | ND | 8.98±0.04 |
| Hs 683 | 1.9±0.2 | 6.11±0.17 | 7.32±0.08 | ND | ND | 8.75±0.10 |
Data are mean values±s.e.m. from three or four individual experiments. Maximal effects are expressed as fold increases above basal cAMP accumulation. Since no maximum was reached for 8-OH-DPAT (see Figure 1), pEC50 values for this agonist are rough estimates. ND, not determined.