Table 2.
Comparison of the effects of cyclosporine, sirolimus, and everolimus on brain metabolism and their presence in brain mitochondria
| Parameter | CsA | SRL | RAD | CsA/SRL | CsA/RAD | Method |
|---|---|---|---|---|---|---|
| Presence in mitochondria | ++ | 0 | + | ++/0 | +/++ | HPLC/MS* |
| Oxid. phosphorylation | ↓ | 0 | ↑ | ↓↓ | 0 | 31P-MRS |
| Krebs cycle (C4-glutamate) | ↓ | 0 | 0 | ↓↓ | 0 | 13C-MRS |
| Glycolysis (C3-lactate) | ↑ | ↓ | ↓ | ↓↓ | 0 | 13C-MRS |
| Intracellular pH | ↓↓ | ↑/0 | 0 | 0 | 0 | 31P-MRS |
| ROS formation | ↑/0 | ↑ | ↑ | ↑↑ | 0 | Fluorescence |
From Serkova et al. (2001). CsA, cyclosporine; HPLC, high-performance liquid chromatography; MRS, magnetic resonance spectroscopy; MS, mass spectrometry; RAD, everolimus; SRL, sirolimus.