Effects of TTX (left upper panel, 3 × 10−6 M, n=8), L-NA (right upper panel, 10−4 M, n=11), atropine (left lower panel, 2 × 10−6 M, n=8) and guanethidine (right lower panel, 10−5 M, n=8) on the EFS (60 V, 1 mS, 2, 4, 8, 16, 32 Hz, 20 S)-elicited contractions. The contractions were expressed as percent of control (the first series). Data represent means±s.e.m. *P<0.05, **P<0.01, ***P<0.001. Comparison was made by ANOVA, followed by Bonferroni post hoc test.