Table 1.

Influence of N^G-nitro-L-arginine (L-NA), tetrodotoxin, atropine, guanethidine, fasudil and Y-27632 on the spontaneous contractile activity of the isolated sheep ureteral helical strips

Control	90.9±2.8%, n=12
L-NAME (10−4 M)	96.2±2.9%, n=7, NS
Control	94.3±2.1%, n=15
TTX (3 × 10−6 M)	93.1±5.2%, n=4, NS
Control	90.9±2.8%, n=12
Atropin (10−6 M)	97.1±2.1%, n=7, NS
Control	76.1±7.5%, n=7
Guanethidine (10−5 M)	89.8±3.8%, n=7, NS
Control	94.3±2.1%, n=15
Y-27632 (10−6 M)	55.7±3.0%***, n=7
Y-27632 (10−5 M)	15.2±3.1%***, n=6
Fasudil (10−6 M)	67.1±3.4%***, n=4
Fasudil (10−5 M)	43.0±2.4%***, n=4

The amplitude of contractions at a given time was expressed as the percentage of the contraction amplitude obtained at time zero. After the application of L-NA and atropine, the spontaneous contractions were evaluated at the end of 20 min; for Y-27632, fasudil and TTX at the end of 15 min and for guanethidine at the end of 60 min. For comparison, one-way ANOVA followed by Bonferroni post hoc test was used.

^***P<0.001, NS: not significant.