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. 2004 Sep 20;143(4):465–476. doi: 10.1038/sj.bjp.0705976

Figure 4.

Figure 4

Suppression of endogenous cAMP production by AS. Myocytes transfected to express GFP (1000 MOI, open bars), AS (1000 MOI, horizontal bars) and SAS (100 MOI ACVI plus 1000 MOI AS, vertical bars) were incubated in buffer containing 100 μM IBMX at 37°C for 10 min. Myocytes were stimulated using 1 μM isoprenaline or 10 μM forskolin in the presence of IBMX for a further 10 min, lysed and their total intracellular cAMP levels were measured. Statistical differences between the three groups in the presence of isoprenaline are indicated by *(one-way ANOVA, P<0.05). Values are means±s.e.m. from six experiments.