Figure 2.

Effects of the I₁R ligand moxonidine (MOX), the I₂BS ligand idazoxan (IDZ) and the I_2ABS ligand amiloride (AM) on the ability of cirazoline (CIR; 60 μM) to potentiate oxidation of benzylamine (300 μM) by BPAO. Moxonidine, idazoxan or amiloride was added to assay wells containing BPAO (0.167 mg ml⁻¹) and either water (−CIR) or cirazoline (+CIR). Plates were warmed to 37°C and reactions were started by the addition of benzylamine. Initial rates of change in absorbance at 498 nm are expressed as a percentage of that in a control experiment containing only BPAO and benzylamine. Data are the mean±s.e. mean values from triplicate determinations; error bars do not exceed symbol size.