Table 2.

Variables determined for clonidine and cirazoline acting to induce two-site nonessential activation and intersecting linear noncompetitive (mixed) inhibition of BPAO, with benzylamine as substrate, by applying a novel equation (see text) to a representative data set (see Figure 8)

Ligand	KH (μM)	α	KL (μM)	β	n	Ki (mM)	γ
Clonidine	7.42±0.82 (8.82)	0.781±0.005 (0.751)	145±10 (164.6)	1.51±0.04 (1.541)	11	19.13	19.8
Cirazoline	6.20±0.39 (5.63)	0.445±0.002 (0.426)	54.1±8.0 (49.0)	1.12±0.01 (1.14)	5	2.85	5.59

Values of K_H, α, K_L and β represent the mean±s.e.m. of variables obtained by analysing n curves, as described in the text. Values for K_i and γ were determined from replots of Dixon plot data (Figure 7), and were held constant during nonlinear regression analyses. Initial estimates for K_H, α, K_L and β obtained from Figures 4 and 5 are shown in parentheses alongside computed values. Values for K_M and V_max in this experiment were determined as 1071 μM and 4.623 mOD min⁻¹, respectively.