Figure 2.

(a) Binding of ³H-JNJ-5207852 to rat H₃ receptor. Membranes from rat brain were incubated with increasing concentrations of ³H-JNJ-5207852. Nonspecific binding was determined in the presence of 10 μM histamine. (b) In vitro autoradiography of histamine H₃ receptors in sagittal sections from mouse brain. Sections from wild-type (H₃^+/+, top) and knockout (H₃^−/−, bottom) mice were incubated with 3 nM ³H-JNJ-5207852 for 10 min prior to washing. Cortex (Cx), striatum (Str), hypothalamus (Hyp) and cerebellum (Cer) are indicated by arrows. Bar indicates 1 cm.