Table 2.

At a low glucose concentration the ATP concentration can become too low to start up glycolysis in the absence of the glycosome

Glycosome	[Glc]out, mM	Cytosolic conc., mM			Glycosomal conc., mM			HK saturation, %
		[ATP]	[ADP]	[AMP]	[ATP]	[ADP]	[AMP]
With	0.01	0.00	0.18	3.82	4.00	0.00	0.00	97
	0.05	0.08	0.74	3.18	3.99	0.01	0.00	97
Without	0.01	0.00	0.00	4.00	—	—	—	0
	0.05	0.06	0.68	3.25	—	—	—	8

The steady-state adenine nucleotide concentrations have been calculated at different extracellular glucose concentrations. The percentage saturation of HK with ATP was calculated as 100%·([ATP]/K_m,ATP^HK)/(1 + [ATP]/K_m,ATP^HK + [ADP]/K_m,ADP^HK), in which K_m,ATP^HK was 0.116 mM and K_m,ADP^HK was 0.126 mM (9). In the absence of a glycosome the cytosolic concentrations of ATP and ADP were used, and if HK was localized in the glycosome, the glycosomal concentrations of these compounds were used.