. 2004 Oct 25;143(6):785–793. doi: 10.1038/sj.bjp.0705993

Table 1.

Effects of various pharmacological agents on EFS (13 Hz, 120 mA, 1 ms, pulse trains of 2 min)-induced relaxant responses of rat gastric fundus strips precontracted with U46619 (0.1 μM) and incubated with atropine (1 μM) and guanethidine (5 μM)

Test agent	n	Relaxation amplitude (% change from control)	Relaxation AUC (% change from control)
D-NAME (1 mM)	8	0±2.6	−0.3±3.1
L-NAME (1 mM, 20 min)	9	−7.2±3.0^*	4.2±4.5
L-NAME (1 mM, 40 min)	5	−12.0±2.8^*	1.9±6.0
Apamin (0.1 μM)	8	1.3±1.7	−15.2±3.8^*
L-NAME (1 mM)+Apamin (0.1 μM)	8	−6.4±4.2	−20.9±5.3^**
L-NAME (1 mM)+α-Chymotrypsin (1 U ml⁻¹)	9	−33.9±3.6^{***, ##, ###}	−69.7±2.8^***
PPADS (100 μM)	6	−3.9±2.3	−7.1±4.1
Desensitization to ATP	9	−16.3±2.8^*	−18.2±5.2^*
Vehicle (time controls for desensitization to ATP)	6	−15.1±4.1^*	−14.7±4.5^*
L-NAME (1 mM)+α-chymotrypsin (1 U ml⁻¹)+PPADS (100 μM)	6	−40.3±2.8^***	−66.7±4.8^***
L-NAME (1 mM)+α-chymotrypsin (1 U ml⁻¹)+desensitization to ATP	6	−36.7±4.0^**	−65.1±2.1^***
L-NAME (1 mM)+α-chymotrypsin (1 U ml⁻¹)+apamin (0.3 μM)	3	−23.3±2.9	−68.8±0.6^*
L-NAME (1 mM)+α-chymotrypsin (1 U ml⁻¹)+ZnPPIX (300 μM)	4	−33.9±4.4^**	−66.1±9.3^**
L-NAME (1 mM)+α-chymotrypsin (1 U ml⁻¹)+ferrous Hb (100 μM)	3	−37.2±3.1^*	−63.7±3.8^*
L-NAME (1 mM)+anti-VIP serum (1 : 100)	7	−23.3±8.9^*	−64.0±3.6^{***, §§§}
D-NAME (1 mM)+normal rabbit serum (1 : 100)	7	1.3±3.4	−20.1±4.7^*
L-NAME (1 mM)+anti-VIP serum (1 : 100)+PPADS (100 μM)	4	−17.8±5.2	−57.6±8.9^*
L-NAME (1 mM)+anti-VIP serum (1 : 100)+apamin (0.1 μM)	9	−63.7±4.9^{***, †††}	−79.9±3.3^{***, †}

Results are presented as means±s.e.m. of n experiments.

Significant differences between test and control responses: ^*P<0.05, ^**P<0.01, ^***P<0.001.

^##

P<0.01 vs L-NAME (1 mM, 40 min);

^###

P<0.001 vs L-NAME (1 mM, 20 min);

^§§§

P<0.001 vs D-NAME (1 mM)+normal rabbit serum (1 : 100);

^†

P<0.05,

^†††

P<0.001 vs L-NAME (1 mM)+anti-VIP serum (1 : 100).