Figure 8.

Effect of pentoxifylline pretreatment on TNF-α expression in skin tissue of hind paw of rats injected with carrageenin. Panel a: Negative control for immunostaining of TNF-α. The skin tissue slice from a rat paw pretreated with saline and injected with carrageenin was incubated in the absence of first anti-rat TNF-α and no staining was obtained. Panel b: absence of immunostaining of TNF-α in skin tissue of rat paw injected with vehicle (saline) alone. Panel c: intense immunostaining of TNF-α in inflammatory cells of rat paw pretreated with vehicle (saline) and stimulated with carrageenin (100 μg paw⁻¹). Panel d: reduction of immunostaining of TNF-α in inflammatory cells of rat paw pretreated with pentoxifylline (9 mg paw⁻¹) and stimulated with carrageenin (100 μg paw⁻¹). DAB and Mayer's hematoxylin.