Figure 5.

Additive blockade of I_Ach by BW284c51 and tacrine. (a) Representative currents evoked in an oocyte upon superfusion either with 100 μM Ach alone or together with 0.5 μM BW285c51 (upper records), 10 μM tacrine (Tac; middle records) or both inhibitors simultaneously (lower panel). As shown, the blockade caused by individual application of these drugs was comparable, but their coapplication resulted in an increased inhibition. (b) Column graph showing the inhibitory effects of BW284c51 and/or tacrine on I_Ach. The height of the bars indicates the fraction of the control I_Ach remaining after the application of Ach with the indicated drugs. Data are the mean±s.e.m. (five oocytes from two donors). Different number of asterisks atop the bars indicate significant differences between two groups (P<0.05, as determined by the analysis of variance on ranks).