Table 1.

BW284c51 effects on I_Ach time to peak and desensitisation

Test	Time to peak (s)	Desensitisation (%)
		2 s	10 s	20s
10 μM Ach (n=34, 7)	7.1±0.5	3±1	14±3	25±3
10 μM Ach+0.5 μM BW284c51 (n=34, 7)	5.2±0.5**	5±1**	17±2*	23±2
100 μM Ach (n=67, 13)	3.4±0.2	14±2	49±2	67±2
100 μM Ach+0.5 μM BW284c51 (n=67, 13)	2.0±0.1**	44±2**	75±1**	83±1**
100 μM Cch (n=21, 6)	9.3±1.1	2±1	12±2	30±4
100 μM Cch+0.5 μM BW284c51 (n=21, 6)	9.4±1.2	4±1*	11±2	26±5
1000 μM Cch (n=13, 2)	3.4±0.2	16±2	55±4	72±3
1000 μM Cch+0.5 μM BW284c51 (n=13, 2)	2.6±0.2**	33±5**	60±5	71±6

Data show desensitisation values for either Ach (10 and 100 μM) or Cch (100 and 1000 μM) currents, elicited by each agonist alone or coapplied with 0.5 μM BW284c51. The time to I_Ach peak data is the time between the current onset and its maximal value. Desensitisation values were obtained using the equation: D_ti=100−((I_ti/I_peak) ^* 100), where D_ti is the desensitisation value at the specified time, I_peak the peak current amplitude, and I_ti the current amplitudes remaining at 2, 10 and 20 s after the peak. Note that desensitisation increased with concentration for both Ach- and Cch-elicited responses, and that BW284c51 mainly enhanced the fast desensitisation component (measured as the 2 s desensitisation), although the slow one (measured as the 20 s desensitisation) was also increased for currents elicited by 100 μM ACh. The numbers of cells tested and donors used for each group are given within parentheses. Values from control and the corresponding BW284c51 groups were compared (^*P<0.05; ^**P<0.005).