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Effects of 17β-estradiol and Rp-diastereomers on U46619-induced contraction. Porcine coronary arterial rings were incubated with (a) Rp-8-Br-cAMPS (50 μM) or (b) Rp-8-Br-cGMPS (10 μM) 20 min prior to administration of 17β-estradiol. (c) Rp-8-Br-cGMPS (10 μM) were added 20 min prior to administration of isoproterenol. U46619 was then added cumulatively. For each treatment group, n=5–7. *P<0.05 vs control group (ANOVA followed by post hoc Dunnett's test).
