Figure 4.

(a) Subcellular distribution of PKC-ɛ. Slides show immunofluorescence staining experiment. Cryocuts of control (left panel), xenon treated (middle panel) or isoflurane treated (right panel) hearts were stained for PKC-ɛ. Stained sections are visualized using a fluorescence microscope 630-fold magnification (excitation: 554 nm; emission: 573 nm). (b) Translocation of PKC-ɛ. Membrane (right panel) and cytosolic (left panel) fraction of control, xenon and isoflurane hearts (each n=6) were immunoblotted using antibodies against PKC-ɛ (upper panel) or α-tubulin (lower panel). α-Tubulin was used as standard in order to test for uniform protein distribution on the blot. One representative Western blot experiment is shown. The histogram presents densitometric evaluation as x-fold average light intensity (AVI). Data show means±s.d. ^*P<0.05 vs control.