Skip to main content
. 2005 Jan 17;144(4):467–476. doi: 10.1038/sj.bjp.0705988

Figure 4.

Figure 4

Role of HO activity in vasomotor responses of intrapulmonary artery rings from 14-day-old pigs; the peak of HO-1 expression. Intrapulmonary arteries were dissected from the lower lobe of lungs harvested from 14-day-old piglets. Ring preparations were mounted in 5 ml organ chambers containing gassed (95% O2 : 5% CO2), warmed (37°C), Krebs–Henseleit solution. Over a 1-h equilibration period, a baseline tension of 1 g was applied. Rings were preconstricted with the thromboxane mimetic U46619 before 30 min incubation with L-NAME (1 × 10−4 M), SnPP (1 × 10−5 M), CrMP (5 × 10−6 M) or the NaOH vehicle. The effects of L-NAME, (a) SnPP and (b) CrMP on pre-constricted tone, and on dilator responses of ring preparations to ACh, were expressed as a % of U46619-induced tone (mean±s.e.m., n=8 from n=4 animals). L-NAME versus control: ***P<0.0001, two-way ANOVA. CrMP versus vehicle: ***P<0.0001, two-way ANOVA.